Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector
نویسندگان
چکیده
منابع مشابه
T cells in the uninjected eye after anterior chamber inoculation of herpes simplex virus type 1.
PURPOSE To investigate T cell infiltration in the posterior segment of the uninjected eye after uniocular anterior chamber inoculation of HSV-1. METHODS The anterior chamber of one eye of euthymic BALB/c mice was injected with 1 x 10(4) plaque-forming units (PFU) to 2 x 10(4) PFU of herpes simplex virus type 1 (HSV-1; KOS strain). All mice were examined for retinitis on day 8 postinoculation ...
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Herpes simplex virus (HSV) latent infection of ganglion neurons follows axoplasmic transport of HSV, probably in the form of nucleocapsid from peripheral sites of infection (e.g. footpad). This raises the possibility that latency is dependent on this particular means of presenting HSV to ganglion neurons. To investigate this, we directly infected ganglia of mice with HSV and evaluated latency. ...
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By ultracentrifugation of 30 ml of highly dilute suspensions of herpes simplex virus (HSV) directly onto monolayer cultures grown in centrifuge tubes, infectivity was significantly greater than without centrifugation. Ultracentrifugation at 20,000 to 25,000 rpm (28,000 to 45,000 X g) for 1.5 to 2.3 h was utilized with good preservation of cultures. With low-speed centrifugation at 3,000 rpm (1,...
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The entirety of any organism is achieved primarily by the regulated expression of the components of its genome. We are the phenotypic result of our genotypic expression. That is, our gene products are integrally responsible for the physical, intellectual and behavioral traits that identify us as individuals. Genes are hereditary material composed of nucleotide sequences that code for the synthe...
متن کاملOcular disease induced in mice by anterior chamber inoculation of herpes simplex virus.
Herpes simplex virus type 1 (HSV-1) inoculated directly into the anterior chamber of the mouse eye induced an acute inflammatory process in both the injected eye and its uninjected (contralateral) counterpart. In the former, a rapid intense inflammatory reaction developed in the anterior segment (cornea, anterior chamber) and anterior portion of the uveal tract (iris and ciliary body). The reti...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2000
ISSN: 0969-7128,1476-5462
DOI: 10.1038/sj.gt.3301185